Characterization of Insulin-Like Growth Factor I Receptors on Madin-Darby Canine Kidney (MDCK) Cell Line
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چکیده
منابع مشابه
Characterization of insulin-like growth factor I receptors on Madin-Darby canine kidney (MDCK) cell line.
Specific insulin-like growth factor I (IGF-I) receptors on the Madin-Darby canine kidney (MDCK) cell line were identified and characterized. [125I]IGF-I specifically bound to the cells, but [125I]insulin bindings to the cells was minimal. Unlabeled IGF-I displaced both the IGF-I and insulin bindings with potencies that were 100 and 10 times as great as insulin. By an affinity labeling technique...
متن کاملMadin-Darby Canine Kidney Epithelial Cell Line
The Madin-Darby canine kidney (MDCK) cell line, derived from distal tubule/ collecting duct, expresses differentiated properties of renal tubule epithelium in culture. We studied the expression of adrenergic receptors in MDCK to examine the role of catecholamines in the regulation of renal function. Radioligand-binding studies demonstrated, on the basis of receptor affinities of subtype-selecti...
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Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate subs...
متن کامل(CFTR)-mediated chloride secretion in type I Madin- Darby canine kidney (MDCK) cell line
word count: 248 11 Corresponding Author: 12 Sunhapas Soodvilai, Ph.D. 13 Department of Physiology 14 Faculty of Science, Mahidol University 15 Rama VI Road, Bangkok 10400 Thailand 16 Phone number: +66-2-2015516 17 Fax number: +66-2-3547154 18 Email:[email protected] 19 Articles in PresS. Am J Physiol Renal Physiol (May 29, 2013). doi:10.1152/ajprenal.00579.2012 Copyright © 2013 by the ...
متن کاملMadin-Darby canine kidney cells display type I and type II insulin-like growth factor (IGF) receptors.
Using affinity cross-linking techniques, we report the presence of type I IGF and type II IGF receptors in Madin-Darby canine kidney cells, a line of cells lacking insulin receptors. The IGF receptors were further characterized by competition binding studies and found to be similar to IGF receptors in other tissue types. In Madin-Darby canine kidney cells, the type I IGF receptor binds IGF-I gr...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1987
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.34.339